cx40 polyclonal antibody  (Boster Bio)

Journal: Respiratory Research

Article Title: An improved precision-cut lung slices technique for pulmonary vasoconstriction

doi: 10.1186/s12931-026-03600-x

Figure Lengend Snippet: Acute hypoxia experiment in PCLS. A , Blood vessel morphology in the control group was recorded under the bright-field microscope at the beginning of perfusion, and 40 mM potassium chloride solution was added after the normoxic perfusion was completed. And the subsequent immunofluorescence staining was conducted to determine CX40 expression (right). B , The vascular morphology image in the hypoxic group was recorded in the same way while hypoxic perfusion solution with 1% oxygen was used at the fifth minute after the normmoxic perfusion, and the final exposure to 40 mM potassium chloride solution. And the subsequent immunofluorescence staining was conducted to determine CX40 expression (right). The vessel area was marked with a red dashed line; scale bar: 100 μm (A and B). Due to coverslip mounting required for immunofluorescence staining, the vascular morphology in 200 μm-thick PCLS exhibited slight morphological changes (A and B, right). C , The statistical comparisons of the alteration of vascular area recorded every minute during the entire 35-minute perfusion process, n=4 for each

Article Snippet: Gelatin (porcine skin, type A, 300 g Bloom, Vetec), Cell Tracker CM-DiI (Yeasen Biotechnology, 40718ES50), low melting point agarose (CAS 9012-36-6), tissue perforator (GLOBALEBIO), Penicillin-Streptomycin (1%, Gibco), WST-1 Cell Proliferation and Cytotoxicity Assay Kit (Biyuntian, C0035), CX40 Polyclonal Antibody (BA1593, Boster, rabbit polyclonal antibody), FITC-conjugated Goat Anti-Rabbit IgG H&L (Servicebio, GB22303), α-SMA specific antibody (Proteintech, 67735-1-Ig, Clone: 1E9A11), Cy3-conjugated Goat anti-Mouse IgG H&L (Proteintech, SA00009-1), and Antifade Mounting Medium (Servicebio, G1401).

Techniques: Control, Microscopy, Immunofluorescence, Staining, Expressing

Journal: Respiratory Research

Article Title: An improved precision-cut lung slices technique for pulmonary vasoconstriction

doi: 10.1186/s12931-026-03600-x

Figure Lengend Snippet: Immunofluorescence and H&E staining images of pulmonary artery and vein in consecutive paraffin sections. A , CX40 (green) and α-SMA (red) were expressed in artery, whereas only α-SMA (red) in vein. B , Venous and arterial tissue demonstrated in hematoxylin-eosin staining in mouse lung tissue sections. Scale bar: 50 μm

Article Snippet: Gelatin (porcine skin, type A, 300 g Bloom, Vetec), Cell Tracker CM-DiI (Yeasen Biotechnology, 40718ES50), low melting point agarose (CAS 9012-36-6), tissue perforator (GLOBALEBIO), Penicillin-Streptomycin (1%, Gibco), WST-1 Cell Proliferation and Cytotoxicity Assay Kit (Biyuntian, C0035), CX40 Polyclonal Antibody (BA1593, Boster, rabbit polyclonal antibody), FITC-conjugated Goat Anti-Rabbit IgG H&L (Servicebio, GB22303), α-SMA specific antibody (Proteintech, 67735-1-Ig, Clone: 1E9A11), Cy3-conjugated Goat anti-Mouse IgG H&L (Proteintech, SA00009-1), and Antifade Mounting Medium (Servicebio, G1401).

Techniques: Immunofluorescence, Staining

Journal: Respiratory Research

Article Title: An improved precision-cut lung slices technique for pulmonary vasoconstriction

doi: 10.1186/s12931-026-03600-x

Figure Lengend Snippet: Immunofluorescence and H&E staining images of consecutive paraffin sections. A , CX40 (green) and α-SMA (red) were expressed in both pulmonary artery and bronchus. B , bronchus and arterial tissue structures demonstrated by hematoxylin-eosin staining in mouse lung tissue sections. Scale bar: 50 μm

Article Snippet: Gelatin (porcine skin, type A, 300 g Bloom, Vetec), Cell Tracker CM-DiI (Yeasen Biotechnology, 40718ES50), low melting point agarose (CAS 9012-36-6), tissue perforator (GLOBALEBIO), Penicillin-Streptomycin (1%, Gibco), WST-1 Cell Proliferation and Cytotoxicity Assay Kit (Biyuntian, C0035), CX40 Polyclonal Antibody (BA1593, Boster, rabbit polyclonal antibody), FITC-conjugated Goat Anti-Rabbit IgG H&L (Servicebio, GB22303), α-SMA specific antibody (Proteintech, 67735-1-Ig, Clone: 1E9A11), Cy3-conjugated Goat anti-Mouse IgG H&L (Proteintech, SA00009-1), and Antifade Mounting Medium (Servicebio, G1401).

Techniques: Immunofluorescence, Staining

Journal: Biomedical Journal

Article Title: Endothelial activation, cell-cell interactions, and inflammatory pathways in postoperative atrial fibrillation following cardiac surgery

doi: 10.1016/j.bj.2024.100821

Figure Lengend Snippet: Connexins expression and apoptosis level in a representative tissue sample from each type of patient: permanent AF, POAF and non-AF. Cx40: connexin 40; Cx43: connexin 43. Connexins can be seen in brown between the junctions of the cells. Apoptosis occurs when the nuclei of cardiomyocytes appear brown.

Article Snippet: After endogenous peroxidase and unspecific background blocking, sections were incubated with the corresponding primary antibody diluted in blocking solution overnight at 4 °C (anti-Cx40 polyclonal Antibody [36–4900, Invitrogen, dilution 1/100] and anti-Cx43 monoclonal Antibody [13–8300, Invitrogen, dilution 1/250]).

Techniques: Expressing

Journal: Biomedical Journal

Article Title: Endothelial activation, cell-cell interactions, and inflammatory pathways in postoperative atrial fibrillation following cardiac surgery

doi: 10.1016/j.bj.2024.100821

Figure Lengend Snippet: Connexin levels. A. Cx40. B. Cx43. ∗ p < 0.05 ∗∗ p < 0.01 ∗∗∗ p < 0.001.

Article Snippet: After endogenous peroxidase and unspecific background blocking, sections were incubated with the corresponding primary antibody diluted in blocking solution overnight at 4 °C (anti-Cx40 polyclonal Antibody [36–4900, Invitrogen, dilution 1/100] and anti-Cx43 monoclonal Antibody [13–8300, Invitrogen, dilution 1/250]).

Techniques:

Journal: iScience

Article Title: CCRR regulate MYZAP-PKP2-Nav1.5 signaling pathway in atrial fibrillation following myocardial infarction

doi: 10.1016/j.isci.2024.111102

Figure Lengend Snippet:

Article Snippet: The antibodies used were PKP2 (1:500, abs136897, Absin, China), TLR2 (1:2000, DF7521, Affinity, America), TLR4 (1:2000, 66350-1, Proteintech, America), MYZAP (1:2000, PA5-21116, Invitrogen, America), CX40 (1:1000, DF13633, Affinity, America), CX43 (1:1000, 26980-1, Proteintech, America), Nav1.5 (1:200, ASC-005, Alomone labs, Israel), Cav1.2 (1:200, ACC-003, Alomone labs, Israel), Kv4.2 (1:200, APC-023, Alomone labs, Israel), β-actin(1:3000, A00702, GenScript, America), β-tubulin(1:2000, WL01931, Wanleibio, China) and GAPDH (1:2000, BA002, Bio-Platform, China).

Techniques: Virus, Recombinant, Bicinchoninic Acid Protein Assay, CCK-8 Assay, Immunoprecipitation, SYBR Green Assay, Software, Sequencing

Journal: Nature Genetics

Article Title: Multi-omic profiling of clear cell renal cell carcinoma identifies metabolic reprogramming associated with disease progression

doi: 10.1038/s41588-024-01662-5

Figure Lengend Snippet: ( a , b ): Boxplot of ( a ) T ex score calculated by ssGSEA algorithm in TJ-RCC tumors and ( b ) proportion of T ex in total T cells in snRNA data. P values were calculated by two-sided Turkey’s test. IM1: n = 22, IM2: n = 27, IM3: n = 36, IM4: n = 15. In boxplots, the central line represents the median value, box limits indicate the interquartile ranges, and the whiskers extend to 1.5 times the interquartile range. ( c ): Heatmap showing difference in pathway scores calculated by ssGSEA per cell between different macrophage sub-clusters. Shown are t-values from a lineal model of Limma via comparing selected subgroups to all the other samples. ( d ): Pearson’s correlation between specific subpopulations. P-values are from two-sided Student’s t test. Cell abundance was estimated by ssGSEA score in each sample of TJ-RCC cohort. The red line shows the linear interpolation of the data. X-axes and Y-axes represent ssGSEA score of specific cell types. ( e ): Boxplot of fibroblast score calculated by ssGSEA algorithm in TJ-RCC tumors. P values were calculated by two-sided Tukey’s test. IM1: n = 22, IM2: n = 27, IM3: n = 36, IM4: n = 15. The central line of the box represents the median value, box limits indicate the interquartile ranges, and the whiskers extend to 1.5 times the interquartile range. ( f ): Heatmap showing Pearson’s correlation between cell abundance in TJ-RCC. Cell abundance was represented by ssGSEA score calculated using marker genes from snRNA-seq. ( g ): Ideogram of histological locations of given cell types. ( h ): IHC staining of DCN and ACTA2 on W5_T. Experiment has been repeated three times. ( i ): Multi-color fluorescence on W5_T, associated to Fig. . White box marks a region lack of fibroblast, while yellow box marks a fibroblast enriched region. Figure magnified the yellow box region. ( j ): Multi-color fluorescence on W5_T. Experiment has been repeated three times. ( k ): IHC staining on W5_T. GJA5 marked arterial vascular. Experiment has been repeated three times.

Article Snippet: The following antibodies were used: anti-human CD8 (Abcam, ab178089; 1:100), anti-human CD31 (Proteintech, 11265-1-AP; 1:2,000), anti-Human DCN (Abcam, ab277636, 1:2,000), anti-human CD3 (Proteintech, 17617-1-AP; 1:1,000), anti-human CD163 (Proteintech, 16646-1-AP, 1:2,000), anti-human CX40 (Bioss, bs-1050R; 1:500) and anti-human a-SMA/ACTA2 (Proteintech, 14395-1-AP; 1:2500).

Techniques: Marker, Immunohistochemistry, Fluorescence